1-  Basics of Tissue Culture

Candidates will learn the following: principles of tissue culture, handling of sterile materials under laminar flow, dealing with various equipments included in a tissue culture lab, identifying different cell, their specific culture media and cryopreservation of cultured cells and revival from liquid nitrogen. Course includes seminars and illustrative practical sessions.

 Duration: 5 days

 Fees: 500 EGP for Egyptians/200 US$ for non Egyptians

 Number of trainees:  6 / course

2-  Hands-on Working with Stem Cells

Regular Courses: include an introduction to stem cells, Mesenchymal stem cells (MSCs) isolation by plastic adherence method, hematopoeitic stem cells(HSCs) isolation using MiniMACs separation kit, stem cells culture, and propagation, stem cells surface markers identification by flowcytometer analysis, cryopreservation of stem cells  and their revival from liquid nitrogen.

 

Duration:  2 weeks

Fees: 3000EGP for Egyptians/ 750 US$ for non-Egyptians

Number of trainee: 3- 4/ course.

Optional Courses

1-    MSCs  in vitro differentiation into osteoblasts.

2-    MSCs  in vitro differentiation into adipocytes.

3-    MSCs  in vitro differentiation intochondrocytes

4-    MSCs  in vitro differentiation into hepatocytes.

5-    Pluripotent gene expression  of stem cells by molecular techniques

6-    Telomere length determination of stem cells by molecular techniques.

3-     Development of Antigen Detection Kits

A- Monoclonal Antibodies Production

Course includes immunization of BALB/c mice with specific antigens, myeloma cells revival from liquid nitrogen, and their culture, fusion of immunesplenocytes with myeloma cells, culture of fused cells in drug selection medium, screening for positive hybridomas by ELISA, cloning of positive hybridomas by limiting dilution method, propagation of single cloned hybridomas, cryopreservation of unused hybridomas and large scale production of monoclonal antibodies

    

Duration: 2 months,

Fees: 5000 for Egyptians/1500 US$ for non- Egyptians

Number of trainees: 2/ course

B- Characterization of Monoclonal Antibodies

Course includes ELISA to test for specificity, determination of MAbs, isotyping of MAbs by ELISA isotyping kit, Immunoelecrophoresis (IEP) for determination of ability of MAb to recognize a repetitive epitope. polyacrylamide gel electrophoresis (PAGE) of antigen followed by enzyme  linkedimmunoelectrotransfer blot technique (EITB) for determination of molecular weight  range of recognized antigen and Identification of the chemical nature of recognized antigen.

Duration: 2 week,

Fees: 1000EGP for Egyptians/ 350 US$ for non-Egyptians

Number of trainees: 3-5 / course

C- Application of Monoclonal Antibodies  in Antigen Detection  

Purification of MAbs from ascitic fluid by different purification methods,  conjugation of MAb to horse-radish peroxidase, standardization of sandwich ELISA for antigen detection using MAbs as antigen capture and antigen detection horse radish peroxidase conjugate and modification of sandwich ELISA by loading of MAbs on gold nanoparticles

Duration: 3 weeks,

Fees: 1500 for Egyptians/500 US$ for non- Egyptians

Number of trainees: 3-5/ course

 D- Hands-on ELISA Technique

Course includes an overview to antigen antibody reactions, principle of ELISA technique and its different types, ELISA performance and results interpretation and how to deal with errors that one may face during practical application of ELISA and their corrective and preventive actions

Duration: 3 days

Fees: 300 for Egyptians/ 150 US$ for non- Egyptians

No of trainees: 5-10/course

  Al l courses include seminars and illustrative practical sessions.

 All techniques and materials to be used in the training courses will be accompanied with detailed protocols

1-Airway Management Workshop

 Theoretical overview on anatomy of airway, airway problems, types of supraglottic devices, technology of fiber-optic endoscopy. Course includes hands on practice on the use of different supraglottic devices and fiber-optic intubation using models.

Duration: 2 days

Fees: 500 EGP for Egyptians /250 US $ for non-Egyptians 

2-Hemodynamic  Monitoring Workshop

 Lectures on basic physical principles and hemodynamic calculations of trans-eosophgeal Doppler, Masimo and Picco apparatuses.  Hands on practice on the three monitors with data interpretation .

 Duration: 2 days

 Fees: 500 EGP for Egyptians /250 US $ for non-Egyptians 

3-Regional Anesthesia Workshop

 Theoretical overview of the spinal vertebrae, epidural and subarachnoid spaces with lectures on local anesthetics pharmacology ( pharmacokinetics and pharmacodynamics)  followed by practical training on localization and injection of spinal, continuous spinal, epidural and caudal analgesia

Duration: 2 days

Fees: 500 EGP for Egyptians /250 US $ for non-Egyptians  

4-Sonar Guided Nerve Block & Venous Access

Duration: 2 days

Fees: 500 EGP for Egyptians /250 US $ for non-Egyptians 

1-     Evaluation of Toxicity of Molluscicides :

The course includes evaluation of the applied and promising molluscicides of chemical and natural origins on both target and non-target organisms and environment

Duration: 5 days

Fees: 1000EGP for Egyptians/125US$ for non Egyptians

2-     Ecological Assessment of  Irrigation Systems:

The course includes assessment of canals and agricultural drainage systems and recording their transmission status for parasitic diseases as schistosomiasis and fascioliasis

Duration: 7 days

Fees: 2000 EGP for Egyptians/250 US$ for non Egyptians

1-    Snails Identification by Using Different Methods

The course includes identification of snails by morphology, anatomy and PCR methods.

2-    Methods of Snails Collection

      from natural habitat, transportation to the lab and examination

for cercarial shedding, identification of cercariae 

3-  Snail Control by Using Biological and Chemical  Methods

4-  Ecological  Data

    a- Chemical  analysis for water sulphides, dissolved oxygen, etc.
    b- Physical  parameters such as temp, ph, turbidity, conductivity, velocity of water.

    c- Identification the flora and fauna.

5-  Cercariometry using 2 methods:

    a- Mice infection 

    b- Centrifugation.

6-  Maintaining and Breeding snails.

7-  Snails Infection and examination for Cercarial Shedding.

8-  Mollsucicidal Activity of Compounds and Plant Extracts at Lab or at Snail Research Station

 Fees/ course: 600EGP for Egyptians/200 US$ for non Egyptians

1-    Snails Identification by Using Different Methods

The course includes identification of snails by morphology, anatomy and PCR methods.

2-    Methods of Snails Collection

      from natural habitat, transportation to the lab and examination

for cercarial shedding, identification of cercariae 

3-  Snail Control by Using Biological and Chemical  Methods

4-  Ecological  Data

    a- Chemical  analysis for water sulphides, dissolved oxygen, etc.
    b- Physical  parameters such as temp, ph, turbidity, conductivity, velocity of water.

    c- Identification the flora and fauna.

5-  Cercariometry using 2 methods:

    a- Mice infection 

    b- Centrifugation.

6-  Maintaining and Breeding snails.

7-  Snails Infection and examination for Cercarial Shedding.

8-  Mollsucicidal Activity of Compounds and Plant Extracts at Lab or at Snail Research Station

 Fees/ course: 3000EGP for Egyptians/500 US$ for non Egyptians

Extraction and Isolation of Active Ingredients from Medicinal Plants and  Evaluation of their Biological Activities

The course includes chromatographic isolation of chemical constituents from bioactive medicinal plants using different methods as HPLC, glass columns, paper chromatography, thin layer chromatography using different adsorbents as silica gel, polyamide, sephadex .

 Identification of the chemical structure of the isolated compound using different spectroscopic analysis as mass spectroscopy, proton and carbon nuclear magnetic resonance, ultraviolet and infrared radiation techniques will also be studied.

Assessment of   the different biological activities as antioxidant techniques, antimicrobial and measuring cytotoxicity using brine shrimp method will also be performed.

Duration:  10 days

Fees:  1000 EGP for Egyptians / 150$ for non-Egyptians

1-    Extraction Methods for Nucleic Acids (DNA and RNA) from Biological Samples

The course includes principles, materiasl and reagents as well as demonstration and practical application of the methods
Duration:  10 days
Fees: 600 EGP for Egyptians/ 600 US$ for non Egyptians

2-    Applications of PCR and Quantitative RT-PCR for Diagnosis of Pathogens:

The course includes definition and principle of PCR and different applications of PCR in diagnosis of infectious diseases, cancer and genetic abnormalities.
Duration:  10days
Fees: 1000 EGP for Egyptians/ 1000 US$ for non Egyptian candidates

3-    Cloning and Expression of Recombinant Proteins:

candidates will learn the method of isolation of the target regions in the RNA/DNA genome by specific primers, cloning of PCR product into an expression vector , transformation of the clone into an appropriate bacterial host cell, induction and expression of the proteins of the cloned genes and purification of the expressed proteins by affinity chromatography
Duration:  10 days
Fees: 1000 EGP for Egyptians/ 1000 US$ for non-Egyptians

4-    Determination of SNPs Associated with Different Genetic Diseases (il 28,  il 4):

candidates will learn the meaning and significance of single nucleotide polymorphism (SNP ,pronounced snip,plural snips)and the analytical methods used  to discover known snips and how to discover novel SNPs including the use of real time PCR
Duration:  10 days
Fees:  600 EGP for Egyptians/ 600 US$ for non-Egyptians

1-Basic Laboratory Hematology

This course is intended to provide the participant with the necessary basic training in hematology  laboratory techniques under the supervision of highly experienced specialists Course topics include: Automated blood cell counters (principles & histograms), Interpretation of CBC, diagnostic approach to anemia, red blood cell morphology, screening tests for hemolytic anemia and screening coagulation Practical training includes: Operating automated blood cell counters, blood smear preparation and staining Microscopic slide examination of various RBCs morphology, ESR, Screening tests of hemolytic anemia: (Reticulocyte staining and count, Coomb's test (direct and indirect), Sickle test, Osmotic fragility test), Morphologic examination of mature and immature WBCs, Morphologic examination of normal and megaloblastic erythropoiesis, Coagulation profile: PT, PTT and bleeding time

Duration: 5 days

Fees: Egyptians 500 EGP /200 US$ for non-Egyptians

2-Principal Blood Bank Techniques:

This course will offer the trainee the essential training in blood bank techniques. With completion of the course, the trainee will be able to carry out these techniques on an experienced level. The course includes training on:

·  Blood groups & Rh testing

·  Blood donation

·  Serological examination of donated blood ( HCV, HBV, HIV& syphilis) by ELISA & chemiluminescence techniques

·  Cross matching procedure (manual & card methods)

·  Preparation of packed RBCs

·  Preparation of FFP

·  Preparation of platelet concentrate

·  Preparation of cryoprecipitate                                                                        

Duration: 5 days                                                                                                                                                 

 Fees: Egyptians 500 EGP/ non-Egyptians 200 US$

3-Thrombosis and Hemostasis:

During this course, participants will acquire clinical and basic knowledge in this field. The course is also eligible for a senior hematologist looking for updated information.

Course topics include:

·  Current concepts of the coagulation system

·  Control of coagulation

·  Hemostasis in patients with impaired liver function

·  Venous thrombosis: manifestations, diagnosis and therapy

·  How to approach a patient with bleeding

·  How to approach a patient with venous thrombosis

·  Platelet disorders

·  DIC identification and diagnosis

·  Novel antithrombotic drugs

Practical training:

Measuring PT, PTT and TT, coagulation factor assays and D- dimer level by semi and fully automated coagulometers.

Duration: 5 days

Fees: Egyptians 750 EGP/ non-Egyptians 300 $

4-Hematopoietic Stem Cell (HSC):

This course covers a wide range of topics from basic science to clinical aspects including HSC biology, separation and use in hematological and non-hematological diseases.

Course topics include:

·  HSC biology

·  Methods of HSC collection

·  Identification of HSC

·  Comparison with other sources of stem cells

·  HSC transplantation in hematological diseases

·  HSC therapy in non-hematological diseases

  Practical training:

·  Separation of HSC by fully automated closed system cell separator

·  Identification of HSC by flow cytometry

Duration: 5 days

Fees: Egyptians 1500 L.E/ non Egyptians 500 $

1-    Immunohistochemical and Cell Image Analysis of Tissue Samples

Duration: 2 weeks

Fees: 1000 EGP for Egyptians /600 US$ for non-Egyptians

2-     Procedures and Technical Points in Cytology and FNA of body fluids.

Duration: 2 weeks:

 Fees: 500 EGP for Egyptians /300 US$ for non-Egyptians

3-    Techniques of Tissue Processing

Course includes techniques of  embedding, fxation, cutting and different stains for routine study.

 Duration: 2 weeks

 Fees: 1000 EGP for Egyptians /400 US$ for non-Egyptians

4-    Techniques and Procedures of Immunohistochemical Stains.

 Duration: 2 weeks:

Fees: 1000EGP for Egyptians /200US$ for non-Egyptians

5-    Techniques and Preparation of Frozen Sections and their Staining.

Duration: 2 weeks

Fees: 1000 EGP for Egyptians /200US$ for non-Egyptians

6-    Cell Image Analysis of Tissue Specimens.

Duration: 2 weeks

Fees: 1000EGP for Egyptians /200US$ for non-Egyptians

7-    Fluorescent in-situ hybridization technique (FISH)

Duration: 2 week 

Fees: 2000EGP for Egyptians /400US$ for non-Egyptians

1-    Electron Microscope in Visualization of Nanoparticles

 The course focuses on the EM techniques and how to examine the nanoparticles by EM

Duration: 6 days

 Fees: 900 EGP for Egyptians /800US $ for non Egyptians

2-    Immunoelectron Labeling in Research and Diagnosis

The course explains the technique of immunoelectron microscopy and its role in evaluation of infectious diseases

Duration: 6 days

Fees: 900 EGP for Egyptians /800US $ for non Egyptians

3-    Value of Electron Microscopy in Research and Early Diagnosis of Disease

 The course focuses on the techniques and personnel experience of the department in research using EM

 Also it highlights the value of EM in the detection of cellular changes for early diagnosis of diseases.

Duration: 6 days 

Fees: 900 EGP for Egyptians /800US $ for non-Egyptians

4-    Electron Microscopy and Parasitic Diseases

 The course focuses on the techniques and the study of parasites using EM 

Duration: 6 days 

Fees: 900 EGP for Egyptians /800US $ for non-Egyptians

1- Microbiological Diagnosis of Infectious Diseases

The course includes training on culture of different clinical specimens
 (urine, stools, pus ,etc.), blood and other body fluids using conventional 
culture techniques and automated systems: Bact/ALERT and BACTEC
 9050; identification of clinical isolates to the species level using the
 Analytical Profile Index (API) and antimicrobial susceptibility testing of isolates

 Duration: 5 days

Fees: 1000 EGP for Egyptians/ $ 250 for non-Egyptians

2- Bacterial and Fungal Cultures on Automated Instruments

Course includes culture, species identification, antimicrobial susceptibility
 testing and recording of the minimum inhibitory concentration (MIC) 
of different antibiotics by VITEK2 automated system”                                                      

 Duration: 5 days

 Fees: 2000 EGP for Egyptians/ $ 400 for non-Egyptians

3- Guidelines on microbiological specimen collection and transport

Duration: 3 days    

 Fees: 300 EGP for Egyptians/ $ 100 for non-Egyptians