Immunology Department

1-  Basics of Tissue Culture

Candidates will learn the following: principles of tissue culture, handling of sterile materials under laminar flow, dealing with various equipments included in a tissue culture lab, identifying different cell, their specific culture media and cryopreservation of cultured cells and revival from liquid nitrogen. Course includes seminars and illustrative practical sessions.

 Duration: 5 days

 Fees: 500 EGP for Egyptians/200 US$ for non Egyptians                                                                         

 Number of trainees:  6 / course

2-  Hands-on Working with Stem Cells

Regular Courses: include an introduction to stem cells, Mesenchymal stem cells (MSCs) isolation by plastic adherence method, hematopoeitic stem cells(HSCs) isolation using MiniMACs separation kit, stem cells culture, and propagation, stem cells surface markers identification by flowcytometer analysis, cryopreservation of stem cells  and their revival from liquid nitrogen.

Duration:  2 weeks                                                                                                                                            

Fees: 3000EGP for Egyptians/ 750 US$ for non-Egyptians

Number of trainee: 3- 4/ course.

Optional Courses

1-    MSCs  in vitro differentiation into osteoblasts.

2-    MSCs  in vitro differentiation into adipocytes.

3-    MSCs  in vitro differentiation intochondrocytes

4-    MSCs  in vitro differentiation into hepatocytes.

5-    Pluripotent gene expression  of stem cells by molecular techniques

6-    Telomere length determination of stem cells by molecular techniques.

3-     Development of Antigen Detection Kits

A-    Monoclonal Antibodies Production

Course includes immunization of BALB/c mice with specific antigens, myeloma cells revival from liquid nitrogen, and their culture, fusion of immunesplenocytes with myeloma cells, culture of fused cells in drug selection medium, screening for positive hybridomas by ELISA, cloning of positive hybridomas by limiting dilution method, propagation of single cloned hybridomas, cryopreservation of unused hybridomas and large scale production of monoclonal antibodies

    

Duration: 2 months,

Fees: 5000 for Egyptians/1500 US$ for non- Egyptians

Number of trainees: 2/ course

B-  Characterization of Monoclonal Antibodies

Course includes ELISA to test for specificity, determination of MAbs, isotyping of MAbs by ELISA isotyping kit, Immunoelecrophoresis (IEP) for determination of ability of MAb to recognize a repetitive epitope. polyacrylamide gel electrophoresis (PAGE) of antigen followed by enzyme  linkedimmunoelectrotransfer blot technique (EITB) for determination of molecular weight  range of recognized antigen and Identification of the chemical nature of recognized antigen.

Duration: 2 week,

Fees: 1000EGP for Egyptians/ 350 US$ for non-Egyptians

Number of trainees: 3-5 / course

C-  Application of Monoclonal Antibodies  in Antigen Detection  

Purification of MAbs from ascitic fluid by different purification methods,  conjugation of MAb to horse-radish peroxidase, standardization of sandwich ELISA for antigen detection using MAbs as antigen capture and antigen detection horse radish peroxidase conjugate and modification of sandwich ELISA by loading of MAbs on gold nanoparticles

Duration: 3 weeks,

Fees: 1500 for Egyptians/500 US$ for non- Egyptians

Number of trainees: 3-5/ course

 D - Hands-on ELISA Technique

Course includes an overview to antigen antibody reactions, principle of ELISA technique and its different types, ELISA performance and results interpretation and how to deal with errors that one may face during practical application of ELISA and their corrective and preventive actions

Duration: 3 days

Fees: 300 for Egyptians/ 150 US$ for non- Egyptians

No of trainees: 5-10/course

·  Al l courses include seminars and illustrative practical sessions.

·  All techniques and materials to be used in the training courses will be accompanied with detailed protocols