1- Basics
of Tissue Culture
Candidates
will learn the following: principles of tissue culture, handling of sterile
materials under laminar flow, dealing with various equipments included in a
tissue culture lab, identifying different cell, their specific culture media
and cryopreservation of cultured cells and revival from liquid nitrogen. Course
includes seminars and illustrative practical sessions.
Duration: 5
days
Fees:
500 EGP for Egyptians/200 US$ for non Egyptians
Number
of trainees: 6 / course
2- Hands-on
Working with Stem Cells
Regular
Courses: include an introduction to stem cells, Mesenchymal stem cells (MSCs)
isolation by plastic adherence method, hematopoeitic stem cells(HSCs) isolation
using MiniMACs separation kit, stem cells culture, and propagation, stem cells
surface markers identification by flowcytometer analysis, cryopreservation of
stem cells and their revival from liquid nitrogen.
Duration: 2
weeks
Fees: 3000EGP for Egyptians/ 750 US$ for
non-Egyptians
Number of
trainee: 3- 4/ course.
Optional
Courses
1- MSCs in
vitro differentiation into osteoblasts.
2- MSCs in
vitro differentiation into adipocytes.
3- MSCs in
vitro differentiation intochondrocytes
4- MSCs in
vitro differentiation into hepatocytes.
5- Pluripotent
gene expression of stem cells by molecular techniques
6- Telomere
length determination of stem cells by molecular techniques.
3- Development
of Antigen Detection Kits
A- Monoclonal
Antibodies Production
Course includes
immunization of BALB/c mice with specific antigens, myeloma cells revival from
liquid nitrogen, and their culture, fusion of immunesplenocytes with myeloma
cells, culture of fused cells in drug selection medium, screening for positive
hybridomas by ELISA, cloning of positive hybridomas by limiting dilution
method, propagation of single cloned hybridomas, cryopreservation of unused
hybridomas and large scale production of monoclonal antibodies
Duration: 2
months,
Fees: 5000
for Egyptians/1500 US$ for non- Egyptians
Number of
trainees: 2/ course
B- Characterization
of Monoclonal Antibodies
Course includes
ELISA to test for specificity, determination of MAbs, isotyping of MAbs by
ELISA isotyping kit, Immunoelecrophoresis (IEP) for determination of
ability of MAb to recognize a repetitive epitope. polyacrylamide gel
electrophoresis (PAGE) of antigen followed by
enzyme linkedimmunoelectrotransfer blot technique (EITB) for
determination of molecular weight range of recognized antigen and
Identification of the chemical nature of recognized antigen.
Duration: 2
week,
Fees: 1000EGP
for Egyptians/ 350 US$ for non-Egyptians
Number of
trainees: 3-5 / course
C- Application
of Monoclonal Antibodies in Antigen Detection
Purification
of MAbs from ascitic fluid by different purification
methods, conjugation of MAb to horse-radish peroxidase, standardization
of sandwich ELISA for antigen detection using MAbs as antigen capture and
antigen detection horse radish peroxidase conjugate and modification of
sandwich ELISA by loading of MAbs on gold nanoparticles
Duration: 3
weeks,
Fees: 1500
for Egyptians/500 US$ for non- Egyptians
Number of
trainees: 3-5/ course
D- Hands-on ELISA Technique
Course
includes an overview to antigen antibody reactions, principle of ELISA
technique and its different types, ELISA performance and results
interpretation and how to deal with errors that one may face during practical
application of ELISA and their corrective and preventive actions
Duration: 3
days
Fees: 300 for
Egyptians/ 150 US$ for non- Egyptians
No of
trainees: 5-10/course
Al
l courses include seminars and illustrative practical sessions.
All
techniques and materials to be used in the training courses will be accompanied
with detailed protocols
